Rasoul Narimani; Mohammad Moghaddam; Nastaran Hemati; Sepideh Mojarab
Volume 3, Issue 1 , November 2017, , Pages 1-8
Abstract
Rosmarinic acid, as one of the most valuable phenolic compounds in different plant species, has severalbiological properties, including antivirus, antibacterial, antitumor and anti-inflammatory. ...
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Rosmarinic acid, as one of the most valuable phenolic compounds in different plant species, has severalbiological properties, including antivirus, antibacterial, antitumor and anti-inflammatory. Elicitors, byproducing messages, can stimulate the production of secondary metabolites in plants. Nitric oxide (abioticelicitor) is a stable gaseous radical, which acts as a signaling molecule in plants and in many physiologicalprocesses, development and environmental stresses. Due to the lack of information on the amount of rosmaricacid and the use of SNP as abiotic elicitor in Nepeta nuda L., the effect of SNP on production of rosmarinicacid was investigated in four concentrations (0, 25, 50 and 100 μM) in vitro shoot culture of catmint hairless.The amount of rosmarinic acid was measured by HPLC after drying samples at room temperature. The resultsof the samples analysis showed that the content of rosmarinic acid increased 37.7% in treatment of nitric oxide(25 μM) compared to the control sample (without application of nitric oxide). Also, amount of rosmarinic acidreduced with increasing nitric oxide concentration in the culture medium. So that the amount of this compoundreached from 54.73 mg.g-1 DW in the control sample to 14.61 mg.g-1 DW in treatment of nitric oxide (100μM). The results of this study indicated catmint hairless plant has the high level of rosmarinic acid and theamount of this valuable drug combination improved with the use of abiotic elicitor nitric oxide at lowconcentration.
